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Electroacupuncture Mimics Exercise-Induced Changes in Skeletal Muscle Gene Expression in Women With Polycystic Ovary Syndrome

Abstract

Context
Autonomic nervous system activation mediates the increase in whole-body glucose uptake in response to electroacupuncture but the mechanisms are largely unknown.

Objective
To identify the molecular mechanisms underlying electroacupuncture-induced glucose uptake in skeletal muscle in insulin-resistant overweight/obese women with and without polycystic ovary syndrome (PCOS).

Design/participants
In a case-control study, skeletal muscle biopsies were collected from 15 women with PCOS and 14 controls before and after electroacupuncture. Gene expression and methylation was analyzed using Illumina BeadChips arrays.

Results
A single bout of electroacupuncture restores metabolic and transcriptional alterations and induces epigenetic changes in skeletal muscle. Transcriptomic analysis revealed 180 unique genes (q < 0.05) whose expression was changed by electroacupuncture, with 95% of the changes towards a healthier phenotype. We identified DNA methylation changes at 304 unique sites (q < 0.20), and these changes correlated with altered expression of 101 genes (P < 0.05). Among the 50 most upregulated genes in response to electroacupuncture, 38% were also upregulated in response to exercise. We identified a subset of genes that were selectively altered by electroacupuncture in women with PCOS. For example, MSX1 and SRNX1 were decreased in muscle tissue of women with PCOS and were increased by electroacupuncture and exercise. siRNA-mediated silencing of these 2 genes in cultured myotubes decreased glycogen synthesis, supporting a role for these genes in glucose homeostasis.

Conclusion
Our findings provide evidence that electroacupuncture normalizes gene expression in skeletal muscle in a manner similar to acute exercise. Electroacupuncture might therefore be a useful way of assisting those who have difficulties performing exercise.

Published in: J Clin Endocrinol Metab. 2020 Jun 1;105(6):dgaa165. doi:10.1210/clinem/dgaa165.

Quelle: https://academic.oup.com/jcem/article/105/6/dgaa165/5813905

Autor: Anna Benrick, Nicolas J Pillon, Emma Nilsson, Eva Lindgren, Anna Krook, Charlotte Ling, Elisabet Stener-Victorin

Kommentar
3x/Woche für 5 Wochen (15 Sitzungen á 30 min); zwei Akupunkturpunkt-Protokolle welche abwechselnd zum Einsatz kamen

Set 1: Ma 29, 32, 34, 36; CV 4, 12; MP 6; Di 4

Set 2: Ma 27; CV 6, 10; MP 10, 6; Le 3; PC 6